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Climacteric Respiration and Fatty and Amino Acids Drive Volatile Ester Biosynthesis in Monthong Durian Aril during Fruit Maturation

Duangkamol Tungsatitporn, Chalermchai Wongs-Aree, Bryl I. Manigo, Wattana Aschariyaphotha, Kitti Bodhipadma, Sompoch Noichinda

Abstract


The distinctive flavor of durian fruit is increasingly characterized, yet the metabolic pathways during ripening are not fully understood. This study investigated the volatile dynamics in ripening Monthong durian, revealing clear correlations between ripening stages and aroma compound production.  Fruit ethylene production peaked one day after the first climacteric peak, initiating the second climacteric peak, pulp softening, and ester and sulfur-containing volatile emission. Ethanethiol, ethyl-2-methyl butanoate, ethyl-2-methyl propanoate, ethyl butanoate, and ethyl hexanoate dominate the odor, along with somewhat fermented ethanol and acetaldehyde. Ethanethiol and ethyl-2-methylbutanoate intensely elevated during late ripening, although D-limonene prevailed early. Short-chain fatty acid esters were performed early on, whereas branched-chain amino acid esters increased significantly in overripe fruit.  Valine, leucine, isoleucine, and methionine increased 1.07–1.29-fold from unripe to overripe, while cysteine and glutamic acid decreased 1.11–1.25-fold. Propionic acid (C3) was found throughout, whereas acetic acid (C2) increased significantly 22-fold from ripe to overripe stages. The main free fatty acids throughout maturation were palmitic (C16:0) and Z-9-oleic (C18:1). Linoleic acid (C18:2) fell by 25% from unripe to ripe pulp, but linolenic acid (C18:3) increased 44%.  Lipoxygenase activity increased throughout maturation, notably in overripe pulp, promoting fatty acid breakdown and volatile generation. From unripe to overripe, aldehyde dehydrogenase and alcohol acetyltransferase activity increased 1.6- and 1.8-fold, respectively. These findings demonstrate that the coordinated metabolism of fatty acids and amino acids, mediated by key enzymes, drives ester biosynthesis during durian fruit ripening.

Keywords



[1]    N. A. W. A. Ali, G. R. Wong, B. C. Tan, W. S. Lum, and P. Mazumdar, “Unleashing the potential of durian: Challenges, opportunities, and the way forward,” Applied Fruit Science, vol. 67, 2025, Art. no. 3, doi: 10.1007/s10341-024-01237-y.

[2]     P. Likhitpichitchai, J. Khermkhan, and S. Kuhaswonvetch, “The competitiveness of Thai durian export to China’s market,” GMSARN International Journal, vol. 17, pp. 371–378, 2023.

[3]     Anonymous. “Demand for durian in the world market is set to surge 400%, Thailand benefits from the popularity of durian in Chaina.” Infoquest.co.th. Accessed: Oct. 13, 2025. [Online.] Available: https://www.infoquest.co.th/ 2023/334785

[4]     B. Jirawut et al., “Postharvest management of durian: The production system and management of quality durians throughout supply chains to chinese market,” Department of Agriculture, Thailand, Ink-On Paper Co. Ltd., Bangkok, 2024.

[5]    W. Aschariyaphotha, C. Wongs-Aree, K. Bodhipadma, and S. Noichinda, “Fruit volatile fingerprints characterized among four commercial cultivars of Thai durian (Durio zibethinus),” Journal of Food Quality, vol. 2021, 2021, Art. no. 1383927, doi: 10.1155/2021/1383927.

[6]    E. Sospeter et al., “Understanding the complex aroma profile of durian fruit: A concise review,” Food Science, vol. 90, 2024, Art. no. e70099, doi: 10.1111/1750-3841.70099.

[7]    W. Phutdhawong, P. Chairat, K. Kantarod, and W. Phutdhawong, “GC-MS and 1 H NMR analysis of fatty acids in Monthong Thai durian (Durio zibethinus Murr),” Chemical Science Transactions, vol. 4, no. 3, pp. 663-667, 2015, doi: 10.7598/cst2015.1068.

[8]    L. Sangpong et al., “Assessing dynamic changes of taste-related primary metabolism during ripening of durian pulp using metabolomic and transcriptomic analyses,” Frontiers in Plant Science, vol. 12, Jun. 2021, Art. no. 687799, doi: 10.3389/fpls.2021.687799.

[9]    A. Wisutiamonkul, S. Promdang, S. Ketsa, and W. G. van Doorn, “Carotenoids in durian growth and postharvest ripening,” Food Chemistry, vol. 180, pp. 301-305, Aug. 2015, doi: 10.1016/ j.foodchem.2015.01.129.

[10]  G. Khaksar, S. Kasemcholathan, and S. Sirikantaramas, “Durian (Durio zibethinus L.): Nutritional composition, pharmacological implications, value-added products, and omics-based investigations,” Horticulturae, vol. 10, no. 4, Mar. 2024, Art. no. 342, doi: 10.3390/ horticulturae10040342.  

[11] S. Mostafa, Y. Wang, W. Zeng, and B. Jin, “Floral scents and fruit aromas: Functions, compositions, biosynthesis, and regulation,” Frontiers in Plant Science, vol. 13, Mar. 2022, Art. no. 860157, doi: 10.3389/fpls.2022.860157.

[12]  J. -X. Li, P. Schieberle, and M. Steinhaus, “Characterization of the major odor-active compounds in Thai durian (Durio zibethinus L. ‘Monthong’) by aroma extract dilution analysis and headspace gas chromatography− olfactometry,” Agricultural and Food Chemistry, vol. 60, no. 45, pp. 11253−11262, Oct. 2012, doi:  10.1021/jf303881k.

[13] F. Yang et al., “Changes and correlation analysis of volatile flavor compounds, amino acids, and soluble sugars in durian during different drying processes,” Food Chemistry: X, vol. 21, Mar. 2024, Art. no. 101238, doi: 10.1016/j.fochx. 2024.101238.

[14] C. Wongs-Aree and S. Noichinda, “Postharvest quality properties of potential tropical fruits related to their unique structural characters,” in Postharvest Handling: A Systems Approach (4th Editions), W. J. Florkowski, N. H. Banks, R. L. Shewfelt, and S. E. Prussia, Eds., Oxford, UK: Academic Press, 2022, pp. 277-316.

[15] S. Noichinda, Y. Ueda, Y. Imahori, and K.  Chachin, “Thioester production and thioalcohol specificity of alcohol acetyltransferase in strawberry fruit,” Food Science and Technology, vol. 5, no. 1, pp. 99–103, 1999, doi: 10.3136/ fstr.5.99.

[16] W. Zhou, W. Kong, C. Yang, R. Feng, and W. Xi, “Alcohol acyltransferase is involved in the biosynthesis of C6 esters in apricot (Prunus armeniaca L.) fruit,” Frontiers in Plant Science, vol. 12, Nov. 2021, Art. no. 763139, doi: 10.3389/fpls.2021.763139.

[17] H. Zhao, D. K. Kosma, and S. Lu, “Functional role of long-chain acyl-CoA synthetases in plant development and stress responses,” Frontiers in Plant Science, vol. 22, Mar. 2021, Art. no. 640996, doi: 10.3389/fpls.2021.640996.

[18] N. X. B. Nguyen, T. Saithong, P. Boonyaritthongchai, M. Buanong, S. Kalapanulak, and C. Wongs-Aree, “Methyl salicylate induces endogenous jasmonic acid and salicylic acid in ’Nam Dok Mai’ mango to maintain postharvest ripening and quality,” Plant Physiology, vol. 303, Dec. 2024, Art. no. 154356, doi: 10.1016/j.jplph.2024.154356.

[19]  Z. Li, T. Hong, Z. Zhao, Y. Gu, Y. Guo, and J.  Han, “Fatty acid profiles and nutritional evaluation of fresh sweet-waxy corn from three regions of China,” Foods, vol. 11, no. 17, Aug. 2022, Art. no. 2636, doi: 10.3390/foods11172636.

[20] L. Lenti, A. Nartea, O. L. Orhotohwo, D. Pacetti, and D. Fiorini, “Development and validation of a new GC-FID method for the determination of short and medium chain free fatty acids in wine,” Molecules, vol. 27, no. 23, Nov. 2022, Art. no. 8195, doi: 10.3390/molecules27238195.

[21] B. T. Amid, H. Mirhosseini, and S. Kostadinović, “Chemical composition and molecular structure of polysaccharide-protein biopolymer from Durio zibethinus seed: Extraction and purification process,” Chemistry Central Journal, vol. 6, Oct. 2012, Art. no. 117, doi: 10.1186/1752-153X-6-117.

[22] M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1–2, pp. 248-254, May 1976, doi: 10.1006/abio.1976. 9999.

[23] L. Sirikesorn, W. Imsabai, S. Ketsa, and W.G. van Doorn, “Ethylene-induced water soaking in Dendrobium floral buds, accompanied by increased lipoxygenase and phospholipase D (PLD) activity and expression of a PLD gene,” Postharvest Biology and Technology, vol. 108, pp. 48-53, Oct. 2015, doi: 10.1016/j.postharvbio. 2015.04.009.

[24] N. Meguro, H. Tsuji, Y. Suzuki, N. Tsutsumi, A. Hirai, and M. Nakazono, “Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following reaeration,” Breeding Science, vol. 56, no. 4, pp. 365–370, 2006, doi: 10.1270/jsbbs. 56.365.

[25] T. Okamura et al., “Characteristics of wine produced by mushroom fermentation,” Bioscience and Bioengineering, vol. 65, no.7, pp. 1596-1600, May 2001, doi: 10.1271/ bbb.65.1596.

[26]  Z.Pang, G. Zhou, J. Chong, X. Dong, and J. Xia, “MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights,” Nucleic Acids Research, vol. 49, no. W1, W388–W396, May 2021, doi: 10.1093/nar/gkab382.

[27]  M. J. Anderson, Permutational Multivariate Analysis of Variance (PERMANOVA). NJ: Wiley, Nov. 2017, pp. 1-15.

[28] S. C. Tongdee, A. Suwanagul, and S. Neamprem, “Durian fruit ripening and the effect of variety, maturity stage at harvest, and atmosphere gases,” Acta Horticulturae, vol. 269, pp. 323-334, 1990, doi: 10.17660/ActaHortic.1990.269.43.

[29] P. Brooncherm and J. Siriphanich, “Postharvest physiology of durian pulp and husk,” Kasetsart Journal (Natural Science), vol. 25, no. 5, pp. 119-125, Dec. 1991.

[30] L. Khurnpoon and J. Siriphanich, “Changes in pectin fractions and enzyme activities during husk dehiscence of Monthong durians,” Acta Horticulturae, vol. 687, pp. 187-192, 2005, doi: 10.17660/ActaHortic.2005.687.22.

[31]  Y. Palapol, S. Kunyamee, M. Thongkhum, S. Ketsa, I. B. Ferguson, and W. G. van Doorn, “Expression of expansin genes in the pulp and the dehiscence zone of ripening durian (Durio zibethinus) fruit,” Plant Physiology, vol. 182, pp. 33-39, Jun. 2015. doi: 10.1016/j.jplph.2015.04. 005.

[32] J. Boonthanakorn, W. Daud, A. Aontee, and C. Wongs-Aree, “Quality preservation of fresh-cut durian cv. ‘Monthong’ using microperforated PET/PE films,” Food Packaging and Shelf Life, vol. 23, Mar. 2020, Art. no. 100452, doi: 10.1016/j.fpsl.2019.100452.

[33] A. Laura, V. Luciano, G. Josep, B. Olga, and M. Montserrat, “Chemical characterization of commercial sherry vinegar aroma by headspace solid-phase microextraction and gas chromatography-olfactometry,” Agricultural and Food Chemistry, vol. 59, no. 8, pp. 4062–4070, Mar. 2011, doi: 10.1021/jf104763u.

[34] N. S. Fischer and M. Steinhaus, “Identification of an important odorant precursor in durian: First evidence of ethionine in plants,” Agricultural and Food Chemistry, vol 68, no. 38, pp. 10397−10402, Dec. 2019, doi: 10.1021/acs.jafc. 9b07065.

[35] V. Gorelova, L. Ambach, F. Rebeille, C. Stove, and D. van Der Straeten, “Folates in plants: Research advances and progress in crop biofortification,” Frontiers in Chemistry, vol. 5, Mar. 2017, Art. no. 21, doi: 10.3389/fchem. 2017.00021.

[36] L. J. van Gemert, Odour Thresholds (revised edition). Utrecht, Netherlands: Oliemans Punter & Partners BV, 2011.

[37] I. Maoz, E. Lewinsohn, and I. Gonda, “Amino acids metabolism as a source for aroma volatiles biosynthesis,” Current Opinion in Plant Biology, vol. 67, Jun. 2022, Art. no. 102221, doi: 10.1016/ j.pbi.2022.102221.

[38] P. Engelgau, S. K. Wendakoon, N. Sugimoto, and R. M. Beaudry, “Fruits produce branched-chain esters primarily from newly synthesized precursors,” Agricultural and Food Chemistry, vol. 73, no. 7, pp. 4196–4207, Feb. 2025, doi: 10.1021/acs.jafc.4c10677.

[39] V. Joshi, J. G. Joung, Z. Fei, and G. Jander, “Interdependence of threonine, methionine and isoleucine metabolism in plants: Accumulation and transcriptional regulation under abiotic stress,” Amino Acids, vol. 39, no. 4, pp. 933–947, Feb. 2010, doi: 10.1007/s00726-010-0505-7.

[40]  L. T. Bui et al., “Conservation of ethanol fermentation and its regulation in land plants,” Experimental Botany, vol. 70, no. 6, pp. 1815–1827, Feb. 2019. doi: 10.1093/jxb/erz052

[41] H.-S. Liao, Y.-H. Chung, and M.-H. Hsieh, “Glutamate: A multifunctional amino acid in plants,” Plant Science, vol. 318, May 2022, Art. no. 111238, doi: 10.1016/j.plantsci.2022.111238.

[42] J. Song and F. Bangerth, “Fatty acids as precursors for aroma volatile biosynthesis in pre-climacteric and climacteric apple fruit,” Postharvest Biology and Technology, vol. 30, no. 2, pp. 113-121, Nov. 2003, doi: 10.1016/S0925-5214(03) 00098-X.

[43]  A. A. Velázquez-López et al., “Lipoxygenase and its relationship with ethylene during ripening of genetically modified tomato (Solanum lycopersicum),” Food Technology and Biotechnology, vol. 58, no. 2, pp. 223–229, Jun. 2020. doi: 10.17113/ftb.58.02.20.6207.

[44]  R. G. der Agopian, J. P. Fabi, and B. R. Cordenunsi-Lysenko, “Metabolome and proteome of ethylene-treated papayas reveal different pathways to volatile compounds biosynthesis,” Food Research International, vol. 131, May 2020, Art. no. 108975, doi: 10.1016/ j.foodres.2019.108975.

[45]  C. Wongs-Aree and S. Noichinda, “Glycolysis fermentative by-products and secondary metabolites involved in plant adaptation under hypoxia during pre- and postharvest (Chapter 4),” in Hypoxia and Annoxia, K. Das and M. S. Biradar, Eds., London, UK: IntechOpen, 2018, pp. 59-72.

[46]  T. Mukherjee, S. Kambhampati, S. A. Morley, T. P. Durrett, and D. K. Allen, “Metabolic flux analysis to increase oil in seeds,” Plant Physiology, vol. 197, no. 2, Feb. 2025, Art. no. kiae595, doi: 10.1093/plphys/kiae595.

[47] H. Zhao, D. K. Kosma, and S. Lu, “Functional role of long-chain acyl-CoA synthetases in plant development and stress responses,” Frontiers in Plant Science, vol. 12, Mar. 2021, Art. no. 640996, doi: 10.3389/fpls.2021.640996.

[48]  W. Aschariyaphotha, S. Noichinda, K. Bodhipadma, and C. Wongs-Aree, “Characterization of alcohol acetyltransferases in the ripe flesh of ‘Monthong’ and ‘Chanthaburi 1′ durians,” Plant Physiology and Biochemistry, vol. 206, Jan. 2024, Art. no. 108241, doi: 10.1016/j.plaphy.2023.108241.

[49] Y. Ueda et al., “Functional characteristics of aldehyde dehydrogenase and its involvement in aromatic volatile biosynthesis in postharvest banana ripening,” Foods, vol. 11, no. 3, 2022, Art. no. 347, doi: 10.3390/foods11030347.

[50] L. Pei et al., “LOX and AAT genes affect the aroma of “Xiaobai” apricot during postharvest storage,” Food Processing and Preservation, vol. 2023, 2023, Art. no. 5558605, doi: 10.1155/ 2023.5558605.

[51]  F. Brian et al., “Ester content of blueberry fruit can be ruled by tailored controlled atmosphere storage management,” Postharvest Biology and Technology, vol. 222, Apr. 2025, Art. no. 113355, doi: 10.1016/j.postharvbio.2024.113355.

[52] X. Zhu et al., “Low temperature storage reduces aroma-related volatiles production during shelf-life of banana fruit mainly by regulating key genes involved in volatile biosynthetic pathways,” Postharvest Biology and Technology, vol. 146, pp. 68–78, Dec. 2018, doi: 10.1016/j.postharvbio.2018.08.015.

[53]  D. Zhou, Q. Liu, C. Wu, T. Li, and K. Tu, “Characterization of soluble sugars, glycosidically bound and free volatiles in fresh-cut pineapple stored at different temperature,” Food Bioscience, vol 43, Oct. 2021, Art. no. 101329, doi: 10.1016/j.fbio.2021.101329.

[54]  P. Rey-Serra, M. Mnejja, and A. Monfort, “Inheritance of esters and other volatile compounds responsible for the fruity aroma in strawberry,” Frontier in Plant Science, vol. 13, Aug. 2022, Art. no. 959155, doi: 10.3389/fpls. 2022.959155.

[55]  Y. Asikin et al., “Assessment of volatile characteristics of okinawan pineapple breeding lines by gas-chromatography–mass-spectrometry-based electronic nose profiling and odor activity value calculation,” Chemosensors, vol. 11, no. 10, Sep. 2023, Art. no. 512, doi: 10.3390/chemosensors11100512.

[56]  Y. Wang et al., “Lipid peroxidation and jasmonic acid involved in the development of soft-nose disorder in ‘Keitt’ mango during storage,” Horticultural Science and Biotechnology, vol. 100, no. 2, pp. 255–264, Aug. 2024. doi: 10.1080/14620316.2024.2395288.

[57] P. Panpetch and S. Sirikantaramas, “Fruit ripening-associated leucylaminopeptidase with cysteinylglycine dipeptidase activity from durian suggests its involvement in glutathione recycling,” BMC Plant Biology, vol. 21, Feb. 2021, Art. no. 69, doi: 10.1186/s12870-021-02845-6.

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DOI: 10.14416/j.asep.2026.02.006

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