Page Header

Optimization, Bioactivity and Composition of Curcama aromatica Salisb. Extraction Oils Extracted by Microwave-assisted and Hydro-distillation

Manat Hearunyakij, Weerachai Phutdhawong


Curcama aromatica Salisb. (C.aromatica) rhizomes were extracted by hydro-distillation (HD) and microwave-assisted extraction (MAE). The response surface method (RSM) and analysis of variance (ANOVA) were carried out to optimize the statistical data of MAE. The extraction oils (Eos) were analyzed by the compositions by GC/MS and were compared to the antioxidant activity, total phenolic contents (TPC), and total flavonoid contents (TFC). The highest yields (dry weight basis) of HD and MAE were 1.96% and 2.22% (w/w) respectively. From the optimization result, the highest yield for MAE was 2.33% at 795 W power, time 44 mins, and a solid/liquid ratio of 100 g/583 ml. The compositions detected from HD and MAE were 41 and 45 contents respectively. Cedrene and copaene were the major compositions, detected in the extraction oils. Cedrene was detected in HD (25.54%) and MAE (23.69%). Copaene was detected in HD (22.97%) and MAE (24.71 %). MAE presented DPPH (%inhibition) at 82.68 ± 0.31 %, TPC at 4227.62 ± 28.72 (mgGAE/g Dry weight), and TFC at 3.92 ± 28.72 (mgRE/g Dry weight) higher than HD. The extraction oils obtained in this study contained many potential compositions, and MAE was an effective method to extract the essential oil from C.aromatica.

Keywords: Cedrene; copaene; response surface method; isolation oil; antioxidant

[1] S. Rajkumari and K. Sanatombi, Nutritional value, phytochemical composition, and biological activities of edible Curcuma species: A review, International Journal of Food Properties, 2017, 20, S2668-S2687.

[2] S.G. Jaiswal and S.N. Naik, Turmeric oil: composition, extraction, potential health benefits and other useful applications, Avicenna Journal of Medical Biochemistry, 2021, 9(2), 93-106.

[3] S. Behura, A. Sahoo, S. Singh, S. Jena, B. Har and S. Nayak, Variation in essential oil yield and volatile constituents of curcuma aromatica rhizome from different regions of eastern and southern India, Journal of Essential Oil Bearing Plants, 2021, 24(6), 1248-1255.

[4] N.S. Dosoky and W.N. Setzer, Chemical composition and biological activities of essential oils of curcuma species, Nutrients, 2018, 10, 1196.

[5] S.V. Nampoothiri, R.M. Phillip, S. Kankangi, C.R. Kiran and A.N. Menon, Essential oil composition, α-amylase inhibition and antiglycation potential of curcuma aromatica salisb, Journal of Essential Oil Bearing Plants, 2015, 18(5), 1051-1058.

[6] H. Xiang, L. Zhang, L. Xi, Y. Yang, X. Wang, D. Lei, X. Zheng and X. Liu, Phytochemical profiles and bioactivities of essential oils extracted from seven Curcuma herbs, Industrial Crops and Products, 2018, 111, 298-305.

[7] S.Y. Tsai, S.J. Huang, C.C. Chyau, C.H. Tsai, C.C. Weng and J.L. Mau, Composition and antioxidant properties of essential oils from curcuma rhizome, Asian Journal of Arts and Sciences, 2011, 2(1), 57-66.

[8] Y. Shi, X. Liang, L. Chi, Y. Chen, L. Liang, J. Zhao, Y. Luo, W. Zhang, Q. Cai, X. Wu, Z. Tan and L. Zhang, Ethanol extracts from twelve Curcuma species rhizomes in China: Antimicrobial, antioxidative and anti-inflammatory activities, South African Journal of Botany, 2021, 140, 167-172.

[9] H. Xiang, L. Zhang, Z. Yang, F. Chen, X. Zheng and X. Liu, Chemical compositions, antioxidative, antimicrobial, anti-inflammatory and antitumor activities of curcuma aromatica Salisb. essential oils, Industrial Crops and Products, 2017, 108,  6-16.

[10] P. Rodsamran and R. Sothornvit, Microwave heating extraction of pectin from lime peel: Characterization and properties compared with the conventional heating method, Food Chemistry, 2019, 278, 364-372.

[11] H.S. Kusuma, D.K.Y. Putri, I. Triesty and M. Mahfud, Comparison of microwave hydrodistillation and solvent-free microwave extraction for extraction of agarwood oil, Chiang Mai Journal of Science, 2019, 46(4), 741-755.

[12] G.F. Najmuldeen, K. Narayanan, D.A. Kirubananthan and G.G. Faisal, Comparison of tongkat ali root chemical composition extracted by soxhlet, conventional steam and microwave assisted extraction techniques, Pharmacognosy Journal, 2018, 10(5), 916-920.

[13] A. Elik, D.K. Yanik and F. Göğüş, Microwave-assisted extraction of carotenoids from carrot juice processing waste using flaxseed oil as a solvent, LWT - Food Science and Technology, 2020, 123, 109100.

[14] S. Rahmati, A. Abdullah and O.L. Kang, Effects of different microwave intensity on the extraction yield and physicochemical properties of pectin from dragon fruit (Hylocereus polyrhizus) peels, Bioactive Carbohydrates and Dietary Fibre, 2019, 18, 100186.

[15] S. Burapan, M. Kim, Y. Paisooksantivatana, B.E. Eser and J. Han, Thai curcuma species: Antioxidant and bioactive compounds, Foods, 2020, 9, 1219.

[16] J. Lim, T.T.H. Nguyen, K. Pal, C.G. Kang,  C. Park, S.W. Kim and D. Kim, Phytochemical properties and functional characteristics of wild turmeric (curcuma aromatica) fermented with Rhizopus oligosporus, Food Chemistry: X, 2022, 13, 100198.

[17], National Institute of Standards and Technology: NIST Chemistry Webbook. Data from NIST Standard Reference Database 69. (Accessed on 15 August 2022)

[18] R.P. Adams, Identification of essential oil components by gas chromatography/mass spectrometry, 4th ed., Allured Publishing Corporation, IL, USA, 2007.

Full Text: PDF

DOI: 10.14416/


  • There are currently no refbacks.