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Isolation and Identification of a Cellulase-producing Bacillus sp. Strain BR0302 from Thai Coastal Wetland Soil

Aiya Chantarasiri, Parima Boontanom, Nattawut Yensaysuk, Puwanat Ajwichai


This study proposed the isolation, screening and identification of cellulase-producing bacteria from Thai coastal wetland soil. Eighty seven bacterial strains which demonstrated cellulase-producing bacteria were investigated for cellulolytic properties. The bacterium identified as Bacillus cereus strain BR0302 exhibited the highest hydrolysis activity on carboxymethylcellulose agar plates. Cellulolytic performance for CMCase activity and FPase activity was 0.121 ± 0.006 and 0.057 ± 0.001 U/mL respectively. Cellulolytic characteristics showed that the B. cereus strain BR0302 could be used for the hydrolysis of cellulosic biomass for biofuel production.


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